Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_6_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_6_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 75321697
Paired-end alignments with a unique best hit 45769591
Pairs without alignments under any condition 17726228
Pairs that did not map uniquely 11825878
Genomic sequence context not extractable (edges of chromosomes) 85

Cytosine Methylation

Total C's analysed 1601850100
Methylated C's in CpG context 21471982
Methylated C's in CHG context 1356883
Methylated C's in CHH context 5810020
Methylated C's in Unknown context 54542
Unmethylated C's in CpG context 186450650
Unmethylated C's in CHG context 277897763
Unmethylated C's in CHH context 1108862802
Unmethylated C's in Unknown context 3983886
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11411558 original top strand
CTOT 11429132 complementary to original top strand
CTOB 11471646 complementary to original bottom strand
OB 11457170 original bottom strand

Deduplication

Alignments analysed 45769506
Unique alignments 37464413
Duplicates removed 8305093
Duplicated alignments were found at 6947758 different positions

Cytosine Methylation after Extraction

Total C's analysed 910669092
Methylated C's in CpG context 11841329
Methylated C's in CHG context 760115
Methylated C's in CHH context 3288745
Unmethylated C's in CpG context 104532651
Unmethylated C's in CHG context 156229733
Unmethylated C's in CHH context 634016519
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot