Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_5_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_5_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 84046138
Paired-end alignments with a unique best hit 51605460
Pairs without alignments under any condition 18775249
Pairs that did not map uniquely 13665429
Genomic sequence context not extractable (edges of chromosomes) 131

Cytosine Methylation

Total C's analysed 1797447404
Methylated C's in CpG context 26305415
Methylated C's in CHG context 1561214
Methylated C's in CHH context 6629878
Methylated C's in Unknown context 63671
Unmethylated C's in CpG context 207531853
Unmethylated C's in CHG context 312507188
Unmethylated C's in CHH context 1242911856
Unmethylated C's in Unknown context 4404409
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12883364 original top strand
CTOT 12873781 complementary to original top strand
CTOB 12919828 complementary to original bottom strand
OB 12928356 original bottom strand

Deduplication

Alignments analysed 51605329
Unique alignments 41935142
Duplicates removed 9670187
Duplicated alignments were found at 8064818 different positions

Cytosine Methylation after Extraction

Total C's analysed 1001853984
Methylated C's in CpG context 14205083
Methylated C's in CHG context 857017
Methylated C's in CHH context 3688422
Unmethylated C's in CpG context 114075011
Unmethylated C's in CHG context 172180736
Unmethylated C's in CHH context 696847715
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot