Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_4_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_4_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 74728031
Paired-end alignments with a unique best hit 45908181
Pairs without alignments under any condition 16768986
Pairs that did not map uniquely 12050864
Genomic sequence context not extractable (edges of chromosomes) 102

Cytosine Methylation

Total C's analysed 1599391484
Methylated C's in CpG context 23340714
Methylated C's in CHG context 1384115
Methylated C's in CHH context 5878620
Methylated C's in Unknown context 57442
Unmethylated C's in CpG context 184831527
Unmethylated C's in CHG context 278458490
Unmethylated C's in CHH context 1105498018
Unmethylated C's in Unknown context 3919106
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11420758 original top strand
CTOT 11501621 complementary to original top strand
CTOB 11533549 complementary to original bottom strand
OB 11452151 original bottom strand

Deduplication

Alignments analysed 45908079
Unique alignments 37692238
Duplicates removed 8215841
Duplicated alignments were found at 6925548 different positions

Cytosine Methylation after Extraction

Total C's analysed 905767036
Methylated C's in CpG context 12781614
Methylated C's in CHG context 771949
Methylated C's in CHH context 3313636
Unmethylated C's in CpG context 103354653
Unmethylated C's in CHG context 155896761
Unmethylated C's in CHH context 629648423
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot