Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_3_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_3_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 78091157
Paired-end alignments with a unique best hit 47623705
Pairs without alignments under any condition 18311792
Pairs that did not map uniquely 12155660
Genomic sequence context not extractable (edges of chromosomes) 94

Cytosine Methylation

Total C's analysed 1670693519
Methylated C's in CpG context 25533290
Methylated C's in CHG context 1462648
Methylated C's in CHH context 6171814
Methylated C's in Unknown context 61366
Unmethylated C's in CpG context 191641924
Unmethylated C's in CHG context 289598207
Unmethylated C's in CHH context 1156285636
Unmethylated C's in Unknown context 4126149
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11910708 original top strand
CTOT 11863283 complementary to original top strand
CTOB 11897437 complementary to original bottom strand
OB 11952183 original bottom strand

Deduplication

Alignments analysed 47623611
Unique alignments 39133069
Duplicates removed 8490542
Duplicated alignments were found at 7141080 different positions

Cytosine Methylation after Extraction

Total C's analysed 966714974
Methylated C's in CpG context 14292801
Methylated C's in CHG context 833216
Methylated C's in CHH context 3552711
Unmethylated C's in CpG context 109585647
Unmethylated C's in CHG context 165674993
Unmethylated C's in CHH context 672775606
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot