Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_10_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_10_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 77576026 Number of paired-end alignments with a unique best hit: 48007674 Mapping efficiency: 61.9% Sequence pairs with no alignments under any condition: 16783783 Sequence pairs did not map uniquely: 12784569 Sequence pairs which were discarded because genomic sequence could not be extracted: 123 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11986262 ((converted) top strand) GA/CT/CT: 11977878 (complementary to (converted) top strand) GA/CT/GA: 12029411 (complementary to (converted) bottom strand) CT/GA/GA: 12014000 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1687130988 Total methylated C's in CpG context: 22194995 Total methylated C's in CHG context: 1322680 Total methylated C's in CHH context: 5572782 Total methylated C's in Unknown context: 51737 Total unmethylated C's in CpG context: 200864245 Total unmethylated C's in CHG context: 297108560 Total unmethylated C's in CHH context: 1160067726 Total unmethylated C's in Unknown context: 4034330 C methylated in CpG context: 10.0% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 16h 12m 30s