FastQCFastQC Report
Fri 26 Sep 2025
SRX18040064_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040064_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35513078
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT875730.24659366332594435Illumina Single End PCR Primer 1 (100% over 50bp)
ATTTGATACAGTAAATTCTCGAACTAATTTCTTTAAGGATGTGATACGCA524230.1476160416171192No Hit
GCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCCTTTAAGTG478080.13462082898024214No Hit
GGAGAAGTTACGCCGGGGATAACAGGCCAATCCTCTAGTAAAGTTCGTAT475320.13384365049968353No Hit
GTTGTTAAGTAACGGTTCTTAAACTTTTAGGTGGTTGAGTATAGGAGTAT423580.11927436985326927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)