FastQCFastQC Report
Fri 26 Sep 2025
SRX18040064_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040064_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35513078
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA1322920.3725162882248618No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC1258610.35440746645503385No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC1002490.28228755615044127No Hit
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT968840.27281217358855797No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT897100.2526111648221537No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC896150.2523436577364542TruSeq Adapter, Index 3 (100% over 50bp)
GCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCA868670.24460566329958783No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT826130.232626977588369No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA755490.21273571386856413No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA689670.19420169662567688No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCA681300.19184481840746104No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT668400.18821235377006745No Hit
CTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAGG663850.18693113562276972No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG623540.1755803875969298No Hit
GGCCATTCTGCTAAAGGTACTGTATAAACAAGCTTCAATAATGATAATGC544650.15336603602762905No Hit
GGCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTC540780.15227629663641096No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT446780.12580717447245773No Hit
CTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGA427390.12034721406012736No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAGGCATTT399550.11250784851710122No Hit
GCTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGT389330.1096300354477863No Hit
CCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACTG374130.10534992207659385No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA373750.10524291924231405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)