FastQCFastQC Report
Fri 26 Sep 2025
SRX18040060_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040060_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24307348
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGAATTAAGGTCGAATTGGATCGAATTGGTTTGTCTCGGGTTGACTTC794300.32677361594526894No Hit
CTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGA653320.2687746931504005No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC590670.24300059389448822No Hit
CTCGGGTTGACTTCAACACCTAGCGAGTGTCAGACACTCCAAGGAGACGC476630.1960847394787782No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA452260.1860589645567258No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG441650.18169402931163037TruSeq Adapter, Index 13 (97% over 39bp)
CCCATACTGAACGCACTGAACTGATCTAACCCGGATCAACTCTCAGAATT319570.13147053310793097No Hit
GTCACTTGGAACGGTGTGATTGATGCCAATTGCTGCGGAAGCATAGGCGA278960.11476365089272593No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG277530.11417535142048404No Hit
CTCGGCTGGATGGGCAGGCCTGCTTGGTGATAAATAGTATGCCATCCCCA268730.11055504697591856No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA265550.10924680059708693No Hit
CTCGTCAAACCATTCATGCAGGCCCACAATCAAAAGGCAAGGAATTTCGC260240.10706227598337753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)