FastQCFastQC Report
Fri 26 Sep 2025
SRX18040054_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040054_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33510306
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC907350.27076744688633997No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG462090.13789489120153067TruSeq Adapter, Index 16 (97% over 39bp)
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA425210.12688932175074738No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA421170.1256837224942082No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT418810.1249794615423685No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC350470.10458573550477276No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTTA345480.10309664137355236No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT338080.10088836550761428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)