FastQCFastQC Report
Fri 26 Sep 2025
SRX18040043_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040043_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29759295
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT908890.305413821127147No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC807000.2711757788616968No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT592030.1989395246090339No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTTA556970.18715833153977607No Hit
CTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAGG525950.17673469751215545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC487460.16380092337536895TruSeq Adapter, Index 9 (100% over 50bp)
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT429850.14444226585340816No Hit
CCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACTG412910.13874992670357278No Hit
CTTCCGTATCCACTGCTGTATCCACCACCGTACCCACCAATGTATCCACC393690.13229144037182333No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA388230.13045671948881854No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC370410.12446867440912157TruSeq Adapter, Index 9 (100% over 50bp)
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA365780.12291285798269079No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTT327070.1099051573634389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)