FastQCFastQC Report
Fri 26 Sep 2025
SRX18040041_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040041_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55418310
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC4864730.8778199840449844TruSeq Adapter, Index 3 (100% over 50bp)
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT2061840.3720503205529003No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC1775900.3204536551186783No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCA1609810.29048341604065514No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA1402230.2530264816808741No Hit
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT1389730.2507709094701733No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC1170910.21128576457853007No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT1123700.20276691945315548No Hit
GCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCA1085220.19582336595973426No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG1034690.1867054408551975No Hit
CTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAGG1033040.18640770532338502No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT990430.17871891077154825No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA815000.1470633081376895No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA774640.1397805165837789No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTC724320.13070048509238194No Hit
GGCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTC704730.1271655523237717No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT654490.11809995649452319No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAGGCATTT633030.11422759012319214No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC626880.11311784859552737TruSeq Adapter, Index 3 (100% over 50bp)
CCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACTG612590.11053927844425426No Hit
CCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTTG598290.10795890383521259No Hit
CCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCAG580720.1047884715358516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)