FastQCFastQC Report
Fri 26 Sep 2025
SRX18040040_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040040_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26854578
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC2300190.8565355225466584TruSeq Adapter, Index 1 (100% over 50bp)
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCA827910.3082938037603868No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT700180.26073021888483966No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA690390.25708465796781466No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC669800.2494174363864515No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC633720.23598211075966266No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTC524690.19538195684921955No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG453920.16902890821818164No Hit
GCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCA418670.15590265466096692No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT396620.14769176413794324No Hit
CTTCCGTATCCACTGCTGTATCCACCACCGTACCCACCAATGTATCCACC367720.1369300981009644No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA346560.12905062220676117No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT335680.12499917146342795No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA308260.11478862188785838No Hit
GGCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTC299470.11151543695827207No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT288840.10755708021179852No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA279130.1039413093737686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)