FastQCFastQC Report
Fri 26 Sep 2025
SRX18040038_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040038_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36960663
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT2510930.6793519910614157No Hit
CTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAGG1999390.5409507940915455No Hit
CCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACTG1513240.40941906263965017No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC1449150.3920790057256278No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTTA1061940.2873162746025416No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT968870.2621354492477584No Hit
CCTACCTAACCATATCCTTAGCAATATTAAGGCTGCACTTAAAAGCATTT965210.26114520727076784No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT934450.2528228457373722No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA876670.23719000928094824No Hit
CCTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAG797150.21567524370436753No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA793690.21473911331081913No Hit
GGCCATTCTGCTAAAGGTACTGTATAAACAAGCTTCAATAATGATAATGC772810.20908986399946344No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA739570.20009651883138566No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTT739510.20008028535635305No Hit
CTGCGGACAAACAGAATCCTACCTAACCATATCCTTAGCAATATTAAGGC710810.19231527313241106No Hit
TGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA549700.1487256870906239No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG531990.14393410637682555No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT502550.13596888129414778No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC465530.12595282719901427No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG385690.10435148308892618TruSeq Adapter, Index 15 (97% over 39bp)
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA379430.10265779052718832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)