FastQCFastQC Report
Fri 26 Sep 2025
SRX18040028_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040028_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30231347
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAAGTTACGCCGGGGATAACAGGCCAATCCTCTAGTAAAGTTCGTAT487380.16121676615997296No Hit
ATTCAACTTTATCTAAAGGAACTCGGCAAATACTAAGCCTCGCCTGTTTA401110.13268016142317443No Hit
CCTGACTTCAGTCGATCATAGGAGAAGTTACGCCGGGGATAACAGGCCAA395460.13081124039891442No Hit
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC394700.1305598457124653No Hit
GCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCTTTTAAGTG385900.12764895986937003No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT363340.12018650707161677Illumina Single End PCR Primer 1 (100% over 50bp)
ATTTGATACAGTAAATTCTCGAACTAATTTCTTTAAGGATGTGATACGCA345230.11419603631951959No Hit
GCTGGTTGTTCAAAAAATGCTTTTAAGTGCAGCCTTAATATTGCTAAGGA334410.11061696986244113No Hit
ATAACATTCAACTTTATCTAAAGGAACTCGGCAAATACTAAGCCTCGCCT327080.10819233426813567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)