FastQCFastQC Report
Fri 26 Sep 2025
SRX18040022_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040022_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36333196
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGA1084930.2986057158307791No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC588960.16209969527591242TruSeq Adapter, Index 11 (100% over 50bp)
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC500020.13762070366724688No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC455730.1254307493345755No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA382060.10515452590518048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)