FastQCFastQC Report
Fri 26 Sep 2025
SRX18040021_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040021_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646715
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCTTTTAAGT652770.2760510286523942No Hit
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC632090.26730562786416634No Hit
GGAGAAGTTACGCCGGGGATAACAGGCCAATCCTCTAGTAAAGTTCGTAT509480.21545487396452317No Hit
CCTGACTTCAGTCGATCATAGGAGAAGTTACGCCGGGGATAACAGGCCAA493980.2089000522905613No Hit
GCTGGTTGTTCAAAAAATGCTTTTAAGTGCAGCCTTAATATTGCTAAGGA432400.182858380117492No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT416840.17627818494027606Illumina Single End PCR Primer 1 (100% over 50bp)
CAAAAAATGCTTTTAAGTGCAGCCTTAATATTGCTAAGGATATGGTTAGG344980.1458891858763469No Hit
CTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGCA338960.14334337771652425No Hit
ATTTGATACAGTAAATTCTCGAACTAATTTCTTTAAGGATGTGATACGCA330160.1396219305725975No Hit
GCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCTTTTAAGTG295000.1247530576657265No Hit
CCTCGATGTTGAATCAGGGATTATACCTTTAAGGCGTAGAAGCTTTAATA261700.11067076335973093No Hit
GTTAGGTAGGATTCTGTTTGTCCGCAGCATTAAGAGTGAGAAGTCTGGCT251760.10646721965397729No Hit
GGTTAGGTAGGATTCTGTTTGTCCGCAGCATTAAGAGTGAGAAGTCTGGC240450.10168431429058962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)