FastQCFastQC Report
Fri 26 Sep 2025
SRX18040021_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040021_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646715
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGA1495980.6326375566331307No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA829320.3507125619774248No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC804230.3401022087000245No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC801710.33903652156335456No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT583630.2468122950693151No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA575900.24354334206675218No Hit
CCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCCT494520.2091284138198477No Hit
TGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA475880.2012457121422574No Hit
CCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCC458370.19384087810928494No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA443520.18756093605390856No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC416940.17632047411236612TruSeq Adapter, Index 10 (100% over 50bp)
CTTATATCAAACTGAATTATAAATTTGTGGCACTATAAACCCTATTTCTC379630.16054238400555848No Hit
CTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAACCT363590.15375920080231018No Hit
CTGAATTATAAATTTGTGGCACTATAAACCCTATTTCTCTTTCTAAAAAT353380.14944147633191332No Hit
GCTCTATTAAACAAATTATTATTTTCAAAAGCTGAAAGACAAGTTACTAA348700.14746234307809775No Hit
CACCATATAGCTATCTTTAGTTGATTGAACAATTTTACTGAGCTCTATTA347730.147052138108824No Hit
GCTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGT320380.13548604954218801No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT301770.1276160346162247No Hit
AGCAGATAGAAACCAACCTGGCTCACGCCGGTCTGAACTCAAATCACGTA298950.12642347996328454No Hit
CTCGTCAAACCATTCATGCAGGCCCACAATCAAAAGGCAAGGAATTTCGC277120.11719175369602078No Hit
ACTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGG268410.11350836680697508No Hit
CGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA249960.10570601455635593No Hit
GTAGGGTATTAAAGGTCGAACAGACCTACTTATTAAAGCTTCTACGCCTT247680.10474182143270216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)