FastQCFastQC Report
Fri 26 Sep 2025
SRX18040018_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040018_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29394521
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1351210.4596809044787632Illumina Single End PCR Primer 1 (100% over 50bp)
GGAGAAGTTACGCCGGGGATAACAGGCCAATCCTCTAGTAAAGTTCGTAT547020.18609590542400742No Hit
CGCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCCTTTAAGT545030.1854189085101948No Hit
CAGAAATTCTGACTAGATTTGACCTGACTTCAGTCGATCATAGGAGAAGT427480.14542846267166593No Hit
GCTGGTTGTTCAAAAAATGCCTTTAAGTGCAGCCTTAATATTGCTAAGGA401530.1366002868357678No Hit
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC367940.12517298716995592No Hit
CAAAAAATGCCTTTAAGTGCAGCCTTAATATTGCTAAGGATATGGTTAGG318770.1084453800080634No Hit
CCTGACTTCAGTCGATCATAGGAGAAGTTACGCCGGGGATAACAGGCCAA318220.10825827030826594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)