FastQCFastQC Report
Fri 26 Sep 2025
SRX18040018_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040018_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29394521
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1359790.46259981579560355TruSeq Adapter, Index 3 (100% over 50bp)
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC1266730.4309408545898741No Hit
CTAGCACTAGCCTGACCTTTAGCCAGACTTCTCACTCTTAATGCTGCGGA1231730.41903387369367234No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA890020.3027843182067842No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC599470.20393936679560112No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT540580.18390502093910632No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA420000.14288377075442052No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT411670.14004990930112451No Hit
GCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCA398110.1354368047024818No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA397100.13509320325376284No Hit
CTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAACCT375190.12763943321274057No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCA339550.11551472466586545No Hit
CCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCCT339530.11550792067678191No Hit
CTCGTCAAACCATTCATGCAGGCCCACAATCAAAAGGCAAGGAATTTCGC336720.114551960210544No Hit
TGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA328090.11161603892099482No Hit
AGCAGATAGAAACCAACCTGGCTCACGCCGGTCTGAACTCAAATCACGTA322400.10968030402672663No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG319130.1085678518115672No Hit
GTAGGGTATTAAAGGTCGAACAGACCTACTTATTAAAGCTTCTACGCCTT318700.10842156604627101No Hit
GCCAGACTTCTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACC307750.10469638202303076No Hit
GGCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTC297780.10130459346488416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)