FastQCFastQC Report
Fri 26 Sep 2025
SRX18040017_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040017_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50703140
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1054040.20788456099563066Illumina Single End PCR Primer 1 (100% over 50bp)
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC685320.1351632265772889No Hit
GGTGGATGGAACACAGGAATTGGTGGAGGCTGGAACACTGGAGTGAGCAC619640.12220939373774485No Hit
CCGGATTGGAAGGTGGATGGAACACAGGAATTGGTGGAGGCTGGAACACT590520.11646615968951823No Hit
CGCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCTTTTAAGT550390.10855146249324993No Hit
CAGGAATTGGTGGAGGCTGGAACACTGGAGTGAGCACTGGACTCATTGGT507330.1000588918161676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)