FastQCFastQC Report
Fri 26 Sep 2025
SRX18040016_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040016_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25125657
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT678460.27002676984725216No Hit
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTTA664870.26461795605981564No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC662590.2637105171021001No Hit
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT624820.24867807436836378No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTT540300.21503915300602883No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG527860.21008803869287876TruSeq Adapter, Index 16 (97% over 39bp)
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA469150.1867214855317017No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC439650.17498049901739884No Hit
CTCGGCATTACGTCTTTAAAAATCTCTTTATTTCGGGAAAAACCATAAGG407190.16206143385623706No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT381210.15172140573279336No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA372240.1481513498333596No Hit
CCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACTG338320.13465120533962555No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT337040.13414176592476765No Hit
CTGCGGACAAACAGAATCCTACCTAACCATATCCTTAGCAATATTAAGGC285250.11352936960016607No Hit
TGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA275160.10951355421273162No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA265450.10564897865158313No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG255820.10181624305386323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)