FastQCFastQC Report
Fri 26 Sep 2025
SRX18040006_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040006_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26725189
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTTAAGTAGCGGTTCTTAAACTTTTAGGTGGTTGAGTATAGGAGTAT451400.16890432468036054No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG408060.15268741410958778Illumina Single End PCR Primer 1 (100% over 50bp)
ATTTGATACAGTAAATTCTCGAACTAATTTCTTTAAGGATGTGATACGCA353740.13236201996550895No Hit
GAAGTTGTTAAGTAGCGGTTCTTAAACTTTTAGGTGGTTGAGTATAGGAG326320.12210203639719816No Hit
CGCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCTTTTAAGT322790.12078118512089848No Hit
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC267740.10018264042959621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)