FastQCFastQC Report
Fri 26 Sep 2025
SRX18040005_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040005_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23780207
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGAAAAGCTAAGCTGAATTAATAAGTAAAAGATTTTTGGGTGGGGCG395810.16644514490559312No Hit
CGCAAAATCGGGCTTTATGATAGCTGGTTGTTCAAAAAATGCCTTTAAGT395280.1662222704789744No Hit
CGCCTAAAGAGTAAAATTTAACCTCTGTTGAATTACAGAAATTCTGACTA335490.14107951204966385No Hit
CCTTAATATTGCTAAGGATATGGTTAGGTAGGATTCTGTTTGTCCGCAGC318250.1338297854177636No Hit
CAGAAATTCTGACTAGATTTGACCTGACTTCAGTCGATCATAGGAGAAGT294450.12382146210922387No Hit
CAAAAACATCACTGAGAGTATTTGATTCTCAGCAGTACCTGCCCAGTGCG277780.11681143061538532No Hit
GGAGAAGTTACGCCGGGGATAACAGGCCAATCCTCTAGTAAAGTTCGTAT269580.1133631847695859No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT255560.10746752540884107Illumina Single End PCR Primer 1 (100% over 50bp)
CAAAAAATGCCTTTAAGTGCAGCCTTAATATTGCTAAGGATATGGTTAGG251320.10568452999589113No Hit
ATTCAACTTTATCTAAAGGAACTCGGCAAATACTAAGCCTCGCCTGTTTA242170.10183679225332226No Hit
CGAGGGCTTTGCTGTCTCTTGATTTTTTATTGAAATTGTAGTGTAGGTGA241220.10143730035655284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)