FastQCFastQC Report
Fri 26 Sep 2025
SRX18040005_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRX18040005_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23780207
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCA1343830.565104416458612No Hit
GTCCTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAA1326370.5577621759137756No Hit
CCCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTAC1282290.5392257519036735No Hit
GCCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGT1145490.4816989187688736No Hit
CCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTC1130600.47543740893424524No Hit
GGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAAC1064680.44771687647630654No Hit
GCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCA702210.2952917945583905No Hit
GCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACT674630.2836939140184945No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAAG670170.28181840469260844No Hit
CTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGTA645450.27142320502088146No Hit
GGCCTGTTATCCCCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTC641780.26987990474599316No Hit
GTGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAA593020.2493754574970689No Hit
CCCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTT482380.20284936964594127No Hit
TGGCACTATAAACCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAA464810.19546087214463692No Hit
CCCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAA447330.18811022124407917No Hit
CCTGATTCAACATCGAGGTGCCAAGCCCTTTAGTTAATACGAACTTTACT427200.17964519821042768No Hit
CCCCAAACTCGCTCCAAGTTATGGTCCGTGTTTATGTTGATAAATTGGTG415430.17469570386834732No Hit
GCTTCTTAGTAATTGTAGGAAAATCTTTCCTATCTTAACGGTCCTTTCGT407040.17116755964319402No Hit
CTTTCGTACATATTAAGATAATATTATTTAAAGCAGATAGAAACCAACCT378580.15919962345155364No Hit
GCCCGATTTTGCGTATCACATCCTTAAAGAAATTAGTTCGAGAATTTACT350390.14734522706215297No Hit
CCGGCGTAACTTCTCCTATGATCGACTGAAGTCAGGTCAAATCTAGTCAG292680.12307714562787447No Hit
CTCACTCTTAATGCTGCGGACAAACAGAATCCTACCTAACCATATCCTTA285820.12019239361541302No Hit
CCCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCC262140.11023453244120204No Hit
CCCACCCAAAAATCTTTTACTTATTAATTCAGCTTAGCTTTTCAAAGTTG259250.1090192360394508No Hit
CCTATTTCTCTTTCTAAAAATTGAAATATTAAAGAAACTAGCACTAGCCT253550.10662228465883412No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC247900.10424635916752112TruSeq Adapter, Index 4 (100% over 50bp)
CGCTAAAATTGTAAACACGGCTCCCATCGATAGCACATAATGAAAATGAC239210.1005920596065459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)