WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3263535
total bases: 299822428
Q20 bases: 293278532(97.8174%)
Q30 bases: 284447595(94.872%)

Read1 after filtering:
total reads: 2391993
total bases: 206892144
Q20 bases: 202278144(97.7699%)
Q30 bases: 196123531(94.7951%)

Filtering result:
reads passed filter: 2391993
reads failed due to low quality: 3419
reads failed due to too many N: 0
reads failed due to too short: 868123
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 28.642%

JSON report: SRX7656994.fastp.json
HTML report: SRX7656994.fastp.html

fastp --in1 SRX7656994.fastq.gz --out1 SRX7656994.fastp.fastq.gz --thread 6 --json SRX7656994.fastp.json --html SRX7656994.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa 
fastp v0.23.4, time used: 10 seconds