WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3440122 total bases: 313025318 Q20 bases: 306180988(97.8135%) Q30 bases: 296983686(94.8753%) Read1 after filtering: total reads: 2439396 total bases: 209185768 Q20 bases: 204552868(97.7853%) Q30 bases: 198425978(94.8563%) Filtering result: reads passed filter: 2439396 reads failed due to low quality: 3655 reads failed due to too many N: 0 reads failed due to too short: 997071 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 23.3904% JSON report: SRX7656992.fastp.json HTML report: SRX7656992.fastp.html fastp --in1 SRX7656992.fastq.gz --out1 SRX7656992.fastp.fastq.gz --thread 6 --json SRX7656992.fastp.json --html SRX7656992.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa fastp v0.23.4, time used: 9 seconds