WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3509111 total bases: 320241634 Q20 bases: 312842476(97.6895%) Q30 bases: 302998764(94.6157%) Read1 after filtering: total reads: 2552338 total bases: 218613273 Q20 bases: 213525316(97.6726%) Q30 bases: 206846467(94.6175%) Filtering result: reads passed filter: 2552338 reads failed due to low quality: 3962 reads failed due to too many N: 0 reads failed due to too short: 952811 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 24.1873% JSON report: SRX7656985.fastp.json HTML report: SRX7656985.fastp.html fastp --in1 SRX7656985.fastq.gz --out1 SRX7656985.fastp.fastq.gz --thread 6 --json SRX7656985.fastp.json --html SRX7656985.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa fastp v0.23.4, time used: 10 seconds