WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2994513
total bases: 287425670
Q20 bases: 281315304(97.8741%)
Q30 bases: 273080676(95.0091%)

Read1 after filtering:
total reads: 2660174
total bases: 229321004
Q20 bases: 224363180(97.838%)
Q30 bases: 217790655(94.972%)

Filtering result:
reads passed filter: 2660174
reads failed due to low quality: 3104
reads failed due to too many N: 0
reads failed due to too short: 331235
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 13.1287%

JSON report: SRX7656983.fastp.json
HTML report: SRX7656983.fastp.html

fastp --in1 SRX7656983.fastq.gz --out1 SRX7656983.fastp.fastq.gz --thread 6 --json SRX7656983.fastp.json --html SRX7656983.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa 
fastp v0.23.4, time used: 11 seconds