WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3575774 total bases: 332752772 Q20 bases: 325592684(97.8482%) Q30 bases: 315821752(94.9118%) Read1 after filtering: total reads: 2781449 total bases: 240777451 Q20 bases: 235535370(97.8229%) Q30 bases: 228490218(94.8969%) Filtering result: reads passed filter: 2781449 reads failed due to low quality: 3951 reads failed due to too many N: 0 reads failed due to too short: 790374 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 28.6723% JSON report: SRX7656980.fastp.json HTML report: SRX7656980.fastp.html fastp --in1 SRX7656980.fastq.gz --out1 SRX7656980.fastp.fastq.gz --thread 6 --json SRX7656980.fastp.json --html SRX7656980.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa fastp v0.23.4, time used: 10 seconds