WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3978118 total bases: 372827989 Q20 bases: 363705950(97.5533%) Q30 bases: 351601262(94.3066%) Read1 after filtering: total reads: 3203292 total bases: 277004621 Q20 bases: 269813188(97.4039%) Q30 bases: 260417128(94.0118%) Filtering result: reads passed filter: 3203292 reads failed due to low quality: 4575 reads failed due to too many N: 0 reads failed due to too short: 770251 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 25.1707% JSON report: SRX7656976.fastp.json HTML report: SRX7656976.fastp.html fastp --in1 SRX7656976.fastq.gz --out1 SRX7656976.fastp.fastq.gz --thread 6 --json SRX7656976.fastp.json --html SRX7656976.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa fastp v0.23.4, time used: 11 seconds