WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 2642269 total bases: 241638992 Q20 bases: 236376108(97.822%) Q30 bases: 229288052(94.8887%) Read1 after filtering: total reads: 1855845 total bases: 159646619 Q20 bases: 156065061(97.7566%) Q30 bases: 151313041(94.78%) Filtering result: reads passed filter: 1855845 reads failed due to low quality: 2634 reads failed due to too many N: 0 reads failed due to too short: 783790 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 24.8164% JSON report: SRX7656973.fastp.json HTML report: SRX7656973.fastp.html fastp --in1 SRX7656973.fastq.gz --out1 SRX7656973.fastp.fastq.gz --thread 6 --json SRX7656973.fastp.json --html SRX7656973.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq_2/polyA.fa fastp v0.23.4, time used: 8 seconds