WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3076413
total bases: 284773921
Q20 bases: 278551485(97.815%)
Q30 bases: 270125205(94.856%)

Read1 after filtering:
total reads: 2375734
total bases: 205901096
Q20 bases: 201343443(97.7865%)
Q30 bases: 195219265(94.8122%)

Filtering result:
reads passed filter: 2375734
reads failed due to low quality: 3394
reads failed due to too many N: 0
reads failed due to too short: 697285
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 25.5078%

JSON report: SRX7656991.fastp.json
HTML report: SRX7656991.fastp.html

fastp --in1 SRX7656991.fastq.gz --out1 SRX7656991.fastp.fastq.gz --thread 6 --json SRX7656991.fastp.json --html SRX7656991.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 9 seconds