WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4120200
total bases: 399104604
Q20 bases: 390488574(97.8412%)
Q30 bases: 378775691(94.9064%)

Read1 after filtering:
total reads: 3704884
total bases: 326678732
Q20 bases: 319421062(97.7783%)
Q30 bases: 309658226(94.7898%)

Filtering result:
reads passed filter: 3704884
reads failed due to low quality: 3812
reads failed due to too many N: 0
reads failed due to too short: 411504
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 25.3974%

JSON report: SRX7656989.fastp.json
HTML report: SRX7656989.fastp.html

fastp --in1 SRX7656989.fastq.gz --out1 SRX7656989.fastp.fastq.gz --thread 6 --json SRX7656989.fastp.json --html SRX7656989.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 12 seconds