WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 4033781 total bases: 362706301 Q20 bases: 354716937(97.7973%) Q30 bases: 344003838(94.8436%) Read1 after filtering: total reads: 2641321 total bases: 224143903 Q20 bases: 219059038(97.7314%) Q30 bases: 212455265(94.7852%) Filtering result: reads passed filter: 2641321 reads failed due to low quality: 5252 reads failed due to too many N: 0 reads failed due to too short: 1387208 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 25.7435% JSON report: SRX7656987.fastp.json HTML report: SRX7656987.fastp.html fastp --in1 SRX7656987.fastq.gz --out1 SRX7656987.fastp.fastq.gz --thread 6 --json SRX7656987.fastp.json --html SRX7656987.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa fastp v0.23.4, time used: 11 seconds