WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3434931 total bases: 326392917 Q20 bases: 319349095(97.8419%) Q30 bases: 309821626(94.9229%) Read1 after filtering: total reads: 2841071 total bases: 248564596 Q20 bases: 242996097(97.7597%) Q30 bases: 235570595(94.7724%) Filtering result: reads passed filter: 2841071 reads failed due to low quality: 3425 reads failed due to too many N: 0 reads failed due to too short: 590435 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 25.1011% JSON report: SRX7656979.fastp.json HTML report: SRX7656979.fastp.html fastp --in1 SRX7656979.fastq.gz --out1 SRX7656979.fastp.fastq.gz --thread 6 --json SRX7656979.fastp.json --html SRX7656979.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa fastp v0.23.4, time used: 9 seconds