WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3766665
total bases: 353809735
Q20 bases: 346362917(97.8952%)
Q30 bases: 336298289(95.0506%)

Read1 after filtering:
total reads: 3005856
total bases: 261822974
Q20 bases: 256148075(97.8325%)
Q30 bases: 248617069(94.9562%)

Filtering result:
reads passed filter: 3005856
reads failed due to low quality: 3745
reads failed due to too many N: 0
reads failed due to too short: 757064
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 26.3331%

JSON report: SRX7656974.fastp.json
HTML report: SRX7656974.fastp.html

fastp --in1 SRX7656974.fastq.gz --out1 SRX7656974.fastp.fastq.gz --thread 6 --json SRX7656974.fastp.json --html SRX7656974.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 9 seconds