WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 6414956 total bases: 586719273 Q20 bases: 573948636(97.8234%) Q30 bases: 556695819(94.8828%) Read1 after filtering: total reads: 4545028 total bases: 387825712 Q20 bases: 379043265(97.7355%) Q30 bases: 367435380(94.7424%) Filtering result: reads passed filter: 4545028 reads failed due to low quality: 8043 reads failed due to too many N: 0 reads failed due to too short: 1861885 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 28.2574% JSON report: SRX7656970.fastp.json HTML report: SRX7656970.fastp.html fastp --in1 SRX7656970.fastq.gz --out1 SRX7656970.fastp.fastq.gz --thread 6 --json SRX7656970.fastp.json --html SRX7656970.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa fastp v0.23.4, time used: 14 seconds