WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3749065 total bases: 338820235 Q20 bases: 331442275(97.8225%) Q30 bases: 321452722(94.8741%) Read1 after filtering: total reads: 2562614 total bases: 218882120 Q20 bases: 213972103(97.7568%) Q30 bases: 207424212(94.7653%) Filtering result: reads passed filter: 2562614 reads failed due to low quality: 4426 reads failed due to too many N: 0 reads failed due to too short: 1182025 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 23.8955% JSON report: SRX7656968.fastp.json HTML report: SRX7656968.fastp.html fastp --in1 SRX7656968.fastq.gz --out1 SRX7656968.fastp.fastq.gz --thread 6 --json SRX7656968.fastp.json --html SRX7656968.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa fastp v0.23.4, time used: 10 seconds