WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2459822
total bases: 223144572
Q20 bases: 218106776(97.7424%)
Q30 bases: 211387094(94.731%)

Read1 after filtering:
total reads: 1716402
total bases: 146427915
Q20 bases: 143026697(97.6772%)
Q30 bases: 138586284(94.6447%)

Filtering result:
reads passed filter: 1716402
reads failed due to low quality: 3349
reads failed due to too many N: 0
reads failed due to too short: 740071
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 26.817%

JSON report: SRX7656967.fastp.json
HTML report: SRX7656967.fastp.html

fastp --in1 SRX7656967.fastq.gz --out1 SRX7656967.fastp.fastq.gz --thread 6 --json SRX7656967.fastp.json --html SRX7656967.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 7 seconds