WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3513359
total bases: 322055749
Q20 bases: 314949976(97.7936%)
Q30 bases: 305420813(94.8348%)

Read1 after filtering:
total reads: 2574095
total bases: 220053637
Q20 bases: 215116595(97.7564%)
Q30 bases: 208610689(94.7999%)

Filtering result:
reads passed filter: 2574095
reads failed due to low quality: 4319
reads failed due to too many N: 0
reads failed due to too short: 934945
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 24.7515%

JSON report: SRX7656965.fastp.json
HTML report: SRX7656965.fastp.html

fastp --in1 SRX7656965.fastq.gz --out1 SRX7656965.fastp.fastq.gz --thread 6 --json SRX7656965.fastp.json --html SRX7656965.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 10 seconds