WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3356121
total bases: 304861878
Q20 bases: 297967865(97.7386%)
Q30 bases: 288805202(94.7331%)

Read1 after filtering:
total reads: 2396469
total bases: 206027330
Q20 bases: 201258611(97.6854%)
Q30 bases: 195019180(94.6569%)

Filtering result:
reads passed filter: 2396469
reads failed due to low quality: 3889
reads failed due to too many N: 0
reads failed due to too short: 955763
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 24.1391%

JSON report: SRX7656964.fastp.json
HTML report: SRX7656964.fastp.html

fastp --in1 SRX7656964.fastq.gz --out1 SRX7656964.fastp.fastq.gz --thread 6 --json SRX7656964.fastp.json --html SRX7656964.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa 
fastp v0.23.4, time used: 9 seconds