WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3821830 total bases: 341631263 Q20 bases: 334129593(97.8042%) Q30 bases: 324137900(94.8795%) Read1 after filtering: total reads: 2550377 total bases: 217703592 Q20 bases: 212805934(97.7503%) Q30 bases: 206437057(94.8248%) Filtering result: reads passed filter: 2550377 reads failed due to low quality: 4798 reads failed due to too many N: 0 reads failed due to too short: 1266655 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 28.0886% JSON report: SRX7656957.fastp.json HTML report: SRX7656957.fastp.html fastp --in1 SRX7656957.fastq.gz --out1 SRX7656957.fastp.fastq.gz --thread 6 --json SRX7656957.fastp.json --html SRX7656957.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --adapter_fasta /mmfs1/gscratch/scrubbed/strigg/analyses/20250415_RNAseq/polyA.fa fastp v0.23.4, time used: 9 seconds