WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 3982247 Q20 bases: 3890758(97.7026%) Q30 bases: 3767911(94.6177%) Read1 after filtering: total reads: 20512 total bases: 1572987 Q20 bases: 1524309(96.9054%) Q30 bases: 1467299(93.2811%) Filtering result: reads passed filter: 20512 reads failed due to low quality: 216 reads failed due to too many N: 0 reads failed due to too short: 29272 reads with adapter trimmed: 656 bases trimmed due to adapters: 5265 reads with polyX in 3' end: 32151 bases trimmed in polyX tail: 1868675 Duplication rate (may be overestimated since this is SE data): 30.882% JSON report: SRX7656996.fastp.json HTML report: SRX7656996.fastp.html fastp --in1 SRX7656996.fastq.gz --out1 SRX7656996.fastp.fastq.gz --thread 6 --json SRX7656996.fastp.json --html SRX7656996.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds