WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4091573 Q20 bases: 4004200(97.8646%) Q30 bases: 3884946(94.9499%) Read1 after filtering: total reads: 21464 total bases: 1683017 Q20 bases: 1635545(97.1794%) Q30 bases: 1578748(93.8046%) Filtering result: reads passed filter: 21464 reads failed due to low quality: 172 reads failed due to too many N: 0 reads failed due to too short: 28364 reads with adapter trimmed: 598 bases trimmed due to adapters: 4756 reads with polyX in 3' end: 30959 bases trimmed in polyX tail: 1863832 Duplication rate (may be overestimated since this is SE data): 28.942% JSON report: SRX7656995.fastp.json HTML report: SRX7656995.fastp.html fastp --in1 SRX7656995.fastq.gz --out1 SRX7656995.fastp.fastq.gz --thread 6 --json SRX7656995.fastp.json --html SRX7656995.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds