WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4158919 Q20 bases: 4067315(97.7974%) Q30 bases: 3943246(94.8142%) Read1 after filtering: total reads: 23762 total bases: 1909965 Q20 bases: 1858034(97.281%) Q30 bases: 1794460(93.9525%) Filtering result: reads passed filter: 23762 reads failed due to low quality: 203 reads failed due to too many N: 0 reads failed due to too short: 26035 reads with adapter trimmed: 515 bases trimmed due to adapters: 4085 reads with polyX in 3' end: 28634 bases trimmed in polyX tail: 1705142 Duplication rate (may be overestimated since this is SE data): 24.594% JSON report: SRX7656994.fastp.json HTML report: SRX7656994.fastp.html fastp --in1 SRX7656994.fastq.gz --out1 SRX7656994.fastp.fastq.gz --thread 6 --json SRX7656994.fastp.json --html SRX7656994.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds