WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4371610 Q20 bases: 4277841(97.855%) Q30 bases: 4150524(94.9427%) Read1 after filtering: total reads: 30775 total bases: 2567314 Q20 bases: 2505149(97.5786%) Q30 bases: 2426518(94.5158%) Filtering result: reads passed filter: 30775 reads failed due to low quality: 154 reads failed due to too many N: 0 reads failed due to too short: 19071 reads with adapter trimmed: 333 bases trimmed due to adapters: 2644 reads with polyX in 3' end: 22626 bases trimmed in polyX tail: 1271835 Duplication rate (may be overestimated since this is SE data): 15.912% JSON report: SRX7656993.fastp.json HTML report: SRX7656993.fastp.html fastp --in1 SRX7656993.fastq.gz --out1 SRX7656993.fastp.fastq.gz --thread 6 --json SRX7656993.fastp.json --html SRX7656993.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds