WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4101757 Q20 bases: 4013050(97.8373%) Q30 bases: 3891971(94.8855%) Read1 after filtering: total reads: 24248 total bases: 1945022 Q20 bases: 1892860(97.3182%) Q30 bases: 1829232(94.0469%) Filtering result: reads passed filter: 24248 reads failed due to low quality: 167 reads failed due to too many N: 0 reads failed due to too short: 25585 reads with adapter trimmed: 576 bases trimmed due to adapters: 4604 reads with polyX in 3' end: 28519 bases trimmed in polyX tail: 1619157 Duplication rate (may be overestimated since this is SE data): 26.798% JSON report: SRX7656992.fastp.json HTML report: SRX7656992.fastp.html fastp --in1 SRX7656992.fastq.gz --out1 SRX7656992.fastp.fastq.gz --thread 6 --json SRX7656992.fastp.json --html SRX7656992.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds