WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4316235 Q20 bases: 4226515(97.9213%) Q30 bases: 4102902(95.0574%) Read1 after filtering: total reads: 28377 total bases: 2336155 Q20 bases: 2279847(97.5897%) Q30 bases: 2208045(94.5162%) Filtering result: reads passed filter: 28377 reads failed due to low quality: 146 reads failed due to too many N: 0 reads failed due to too short: 21477 reads with adapter trimmed: 380 bases trimmed due to adapters: 3062 reads with polyX in 3' end: 24909 bases trimmed in polyX tail: 1442344 Duplication rate (may be overestimated since this is SE data): 17.968% JSON report: SRX7656991.fastp.json HTML report: SRX7656991.fastp.html fastp --in1 SRX7656991.fastq.gz --out1 SRX7656991.fastp.fastq.gz --thread 6 --json SRX7656991.fastp.json --html SRX7656991.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds