WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4071662 Q20 bases: 3984773(97.866%) Q30 bases: 3864562(94.9136%) Read1 after filtering: total reads: 23605 total bases: 1886599 Q20 bases: 1837002(97.3711%) Q30 bases: 1775139(94.092%) Filtering result: reads passed filter: 23605 reads failed due to low quality: 168 reads failed due to too many N: 0 reads failed due to too short: 26227 reads with adapter trimmed: 585 bases trimmed due to adapters: 4687 reads with polyX in 3' end: 29157 bases trimmed in polyX tail: 1651841 Duplication rate (may be overestimated since this is SE data): 30.142% JSON report: SRX7656990.fastp.json HTML report: SRX7656990.fastp.html fastp --in1 SRX7656990.fastq.gz --out1 SRX7656990.fastp.fastq.gz --thread 6 --json SRX7656990.fastp.json --html SRX7656990.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 1 seconds