WARNING: you specified the options for cutting by quality, but forogt to enable any of cut_front/cut_tail/cut_right. This will have no effect. Read1 before filtering: total reads: 50000 total bases: 4601750 Q20 bases: 4505001(97.8976%) Q30 bases: 4372346(95.0149%) Read1 after filtering: total reads: 36283 total bases: 3130217 Q20 bases: 3058164(97.6981%) Q30 bases: 2963828(94.6844%) Filtering result: reads passed filter: 36283 reads failed due to low quality: 138 reads failed due to too many N: 0 reads failed due to too short: 13579 reads with adapter trimmed: 257 bases trimmed due to adapters: 1956 reads with polyX in 3' end: 15909 bases trimmed in polyX tail: 947442 Duplication rate (may be overestimated since this is SE data): 15.806% JSON report: SRX7656989.fastp.json HTML report: SRX7656989.fastp.html fastp --in1 SRX7656989.fastq.gz --out1 SRX7656989.fastp.fastq.gz --thread 6 --json SRX7656989.fastp.json --html SRX7656989.fastp.html --cut_mean_quality 30 --trim_front1 10 --trim_front2 10 --reads_to_process 50000 --trim_poly_x --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT fastp v0.23.4, time used: 0 seconds